ISSN 0021-3454 (print version)
ISSN 2500-0381 (online version)
Menu

4
Issue
vol 67 / April, 2024
Article

DOI 10.17586/0021-3454-2022-65-8-575-580

UDC 535.31, 681.72

DEVELOPMENT OF HIGH-APERTURE MICROSCOPE OBJECTIVES WITH AN ENLARGED FIELD OF VIEW AND IMAGE RECORDING ON CCD DETECTORS

A. D. Kozhina
ITMO University, Faculty of Engineering Research;


E. . Tsyganok
ITMO University, Faculty of Engineering Research; Associate Professor


Read the full article 

Abstract. The aim of the work is to improve light microscopes for biological research, which record images on CCD detectors, providing operation without time spent on optics refocusing. The prospects and relevance of the development of high-aperture microscope objectives with an enlarged field of view, operating in conjunction with a matrix CCD image detector, are shown. A high-aperture wide-field microscope objective has been developed, its optical characteristics are given, and the optical scheme is demonstrated. Evaluation and calculation of tolerances for subsequent manufacturing by the Monte Carlo method are carried out.
Keywords: high-aperture objective, microscopy, blood test, aberration correction, micro-objective, field of view

References:
  1. Andreev L.N., Tsyganok H.A., Kozhina A.D., Soshnicova J.B. Proc. of SPIE, 2020, vol. 11548, DOI: 10.1117/12.2573711.
  2. Baigishieva N.D., Bagomedova N.V., Baigishieva A.A. Bulletin of the Ivanovo Medical Academy, 2019, no. 1(24), рр. 47–50. (in Russ.)
  3. Voitsekhovskaya I.V., Kozlova I.V., Suntsova O.V., Lisak O.V., Doroshchenko E.K., Dzhioev Yu.P. News of the Irkutsk State University. Series: Biology. Ecology, 2014, no. 8, pp. 56-65. (in Russ.)
  4. Musyrgalina F.F. Meditsinskaya parazitologiya (Medical Parasitology), Ufa, 2018, 279 р. (in Russ.)
  5. High Performance ZEISS Objectives for Microscopy, https://www.zeiss.com/microscopy/int/products/microscope-components/objectives.html.
  6. https://azimp.ru/thorlabs/microscope-objectives/. (in Russ.)
  7. Frolov D.N., Vinogradova O.A., Frolov V.N. Proc. SPIE, Optics, Photonics, and Digital Technologies for Imaging Applications V, 2018, vol. 10679, doi: 10.1117/12.2306504.
  8. Frolov D.N., Vinogradova O.A., Wen L., Jing F. Proc. SPIE, Optoelectronic Devices and Integration VII, 2018, vol.10814, DOI: 10.1117/12.2500425.
  9. Frolov D.N., Vinogradova O.A., Frolov V.N., Vakulov P.S. Proc. SPIE, Optical Design and Engineering VII, 2018, vol. 10690, DOI: 10.1117/12.2311799.
  10. ZEISS Axio Zoom.V16 for Biology, https://www.zeiss.com/microscopy/ int/products/stereo-zoom-microscopes/axio-zoom-v16.html.
  11. Hoegele A., Winterot J. Proc. SPIE, Zoom Lenses IV, 2012, vol. 8488.
  12. Hoegele A. Proc. SPIE, Zoom Lenses VI, 2019, vol. 11106.
  13. https://micromed-spb.ru/products/stereomikroskopy/Mikroskop-Mikromed-MS_5_ZOOM-LED/. (in Russ.)
  14. Levenhuk ZOOM 1T Trinocular Microscope, https://www.levenhuk.com/ catalogue/microscopes/levenhuk-zoom-1t/#.YLkTjFAmzcc.
  15. Andreev L.N., Ezhova V.V., Tsyganok E.A., Kozhina A.D. Journal of Optical Technology, 2021, no. 4(88), pp. 175–177.
  16. Andreev L.N., Tsyganok H.A., Ezhova V.V., Kozhina A.D. Proceedings of SPIE, 2020, Vol. 11548, pp. 115480C.
  17. Slyusarev G.G. Metody sinteza opticheskikh sistem (Methods for the Synthesis of Optical Systems), Leningrad, 1975. (in Russ.)